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Online course (C2)

Analysis of sanitation flows

Our course is designed to enable you to distinguish between different sanitation flows or streams through their physicochemical and microbiological characteristics. We will cover waste and sanitation, make a comparison of sanitation flows, look at faecal sludge characteristics, and learn why it is important to measure the characteristics of sanitation flows. After this course you will be able to compare the characteristics of different sanitation flows and assess their potential pollution and health impacts, and evaluate the results gained from laboratory tests to identify samples taken from various sanitation flows. You will have a deep understanding of the critical elements of analysing sanitation flows, and you will be equipped with the knowledge and skills you need to apply them in your own work.

Course introduction video

Course information

  • Objectives

    After completion of the course you will be able to:

    • Identify the range of health hazards related to human excreta in communities;
    • Identify disease and transmission routes associated with human excreta;
    • Evaluate control and prevention of health hazards associated with human excreta.
  • Syllabus

    This course covers the following topics:

    • Introduction to public health
    • Human health hazards and human excreta
    • Review and assessment of transmission routes
    • Characteristics and lifecycles of sanitation-relevant pathogens
    • Control measures
    • Risk evaluation tools
  • Delivery method

    Each course in this series consists of video lectures, presentation slides, reading materials, exercises, and assignments. In principle, each video presentation has its own complementary presentation slides. Some parts of the course also include exercises and/or assignments. Please note that this course is free, open access, and does not include guidance, exams, assessment, or certificate. As a rule of thumb, if you spend 8 hours per week following this course, you should be able to complete the curriculum within 10 weeks.

    Instructions on how to use the course materials are available here.


  • Organization

    Each course has its own coding system. The code of this course is C 2. Each course consists of units. Each unit has a unique code, for example, U 2.1. In the section ‘Unit 2.1 videos │ …” you can find and watch all video-recorded lectures that belong to this unit. Similarly, in the section ‘Unit 2.1 course materials│ …”  you can download all materials that make this unit (e.g. high-resolution videos, presentation slides, reading materials, exercises, assignments, etc) as a single compressed file (in zip format). Some files may be of considerable size, so you need to assure that your computer has sufficient bandwidth and capacity for downloading.

Unit 2.1 videos │ Introduction to sanitation flows

Here you can watch all video-recorded lectures of this unit.

What is waste?

U 2.1.3

1 of 4

What goes into sanitation systems?

U 2.1.9

2 of 4

Comparison of sanitation flows

U 2.1.12

3 of 4

Why to measure?

U 2.1.15

4 of 4

Unit 2.1 course materials │ Introduction to sanitation flows

In this section, you can download all video-recorded lectures, presentations, and other documents that make up this unit.

Unit 2.2 videos │ Laboratory induction

Health & Safety in a faecal sludge laboratory

U 2.2.1

1 of 5

IHE Delft feacal sludge laboratory tour

U 2.2.3

2 of 5

Faecal sludge transport, preservation and preparation

U 2.2.4

3 of 5



4 of 5

Serial dilution


5 of 5

Unit 2.2 course materials │ Laboratory induction

In this section, you can download all video-recorded lectures, presentations, and other documents that make up this unit.

Unit 2.3 videos │ Solids and organics

Introduction to solids and moisture content

U 2.3.1

1 of 4

Analysis of solids and moisture content

U 2.3.3

2 of 4

Introduction to organics

U 2.3.7

3 of 4

COD analysis

U 2.3.10

4 of 4

Unit 2.3 course materials │ Solids and organics

Unit 2.4 videos │ Microbiology

Introduction to microbiology of faecal sludge

U 2.4.1

1 of 2

E.coli analysis

U 2.4.2

2 of 2

Unit 2.4 course materials │ Microbiology

Unit 2.5 videos │ Nutrients

Introduction to nutrients in faecal sludge

U 2.5.1

1 of 3

Total Nitrogen (TN) analysis

U 2.5.4

2 of 3

Total Phosphorus (TP) analysis

U 2.5.5

3 of 3

Unit 2.5 course materials │ Nutrients

Download full package

Analysis of sanitation flows ()

(1,619.4 MB)

Here you can download all the course materials in one zipped file including video-recorded lectures, presentations, study materials, and assignments, in case you wish to do so. Note: the file is large, and in case you have a slow internet connection, it is advisable to download units individually using the above-provided links, instead of attempting to download the full package at once.


Dr. Claire Furlong

Course resource person